dextran hydrodynamic radius

With FITC-dextran 20 K, cumulative 2003;10:33847. Table1 shows TEWL values measured immediately after the microporation of human and pig skins. From Table 1, It is worth noting that the value of 2 is above 0.95, being an acceptable value for viscosimetric measurement. For a limited temperature range, the viscosity of a polymer solution varies generally following a relation similar to that of usual liquids (rrhenius plot), where the polymer concentration is not very high and that temperature remains far enough from glass transition [38], and for system PEAorganic solvents see reference [63]. Multiple Linear Least-Squares Fits with a Common Intercept: Determination of the Intrinsic Viscosity of Macromolecules in Solution. As intrinsic viscosity of dextrans decreases with an increase in solution temperature; chain flexibility enhances with an increase in temperature (see Table 4) showing that the intrinsic viscosity of dextran (with different molecular weights Mw from 8.8 to 200kDa) decreases linearly with an temperature increase in solution, but change to high molecular weight to hyperbranched macromolecule. Correspondence to (1999). Carbohydrate Polymers 95 360-365. These values indicate for dextrans in dilute aqueous solution, both methods give very similar results of the intrinsic viscosity and the respective Mw. Copyright 2006-2023 Thermo Fisher Scientific Inc. All rights reserved, Dextran, Tetramethylrhodamine, 2,000,000 MW, Lysine Fixable, Sulen und Kartuschen fr die Chromatographie, Kunststoffartikel und Zubehr fr das Labor, Spektroskopie, Element- und Isotopenanalyse, Alle Themen fr Hilfe und Support anzeigen, Status und Nachverfolgung von Bestellungen, Reagenzien und Kits fr die Neurowissenschaft, Discover how to track and trace cells by imaging , Hier finden Sie unsere brigen Tracking-Produkte fr Sugetier-Zellkulturen, Molecular Probes Dextran-Konjugate Tabelle14.4. 56 The linkage that the enzyme dextranase breaks down is indicated by the black arrow. Journal of Applied Polymer Science, 13, 2031-2032. (2004). 2016;47:15964. For a rigid or rod like polymer molecule that is greatly extended in solution, the Mark-Houwink a constant approaches to a value of 2.0 [48].The hydrodynamic radius (RH), for a sphere ((a/b) = 2.5) is given by the Einstein relation [49]. Ionic Polymers Based on Dextran: Hydrodynamic Properties in Aqueous Solution and Solvent Mixtures. Materiaux 1-5. Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. Recent developments in polysaccharide-based materials used as adsorbents in wastewater treatment. Int. The Viscosity of Dilute Solutions of Long-Chain Molecules. Bahary, W.S. Webdextran hydrodynamic radius. National Library of Medicine 2012;29(7):201729. 2017 Dec 12;27(1):73-78. doi: 10.1007/s10068-017-0274-0. The dextran has the advantage in terms of low cytotoxicity, excellent biocompatibility, and water-solubility. Analytical Chemistry. Protein Hydrodynamics with Light Scattering Figure4 shows the histological analysis of micropores in human cadaver skin. 2011;16(2):518. Polymer molecular weight distribution. Bohdanecky, Miloslav (1996). Google Scholar. ; Hogan, M.P. Intrinsic viscosities of polyelectrolytes in the absence and in the presence of extra salt: Consequences of the stepwise conversion of dextran into a polycation. ; van Nostrum, C.F. Viswanath, D.S. The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC dextran measured in plasma 1 h after its oral application is a marker of small intestinal permeability. WebR H Hydrodynamic radius [m] The basic DLS setup. Mark-Houwink values confirm that for these conditions dextrans behave as rigid spheres with a tendency at compaction as temperature increases. Theory, Estimation, Experiment, and Data. @ Nilimesh See attached. Dextrans in Aqueous Solution. Experimental Review on Intrinsic Synthesis and characterization of blue dextrans. Drug deliv. Molecular weight (Mv) and hydrodynamic properties can be calculated from there. De Belder, A.N. After 24h, the cumulative permeation of FITC-dextran M.W. The skin poration was performed using the microporation device (LG electronics, Korea). WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). In addition, the permeation rate was higher in pig skin than in human skin. Radiofrequency is a well-known medical technique with expanded application in electrosurgery for laparoscopic medical procedures and in dermatology, for sebaceous glands ablation in acne treatment [12, 13], and treatment of photoaging skin symptoms, including wrinkles and hyperpigmentation [14]. 151-166. Food Sci Biotechnol. The parameters of Mark-Houwink for polymers can vary with the polymer solution and temperature. Format), Citation-(BibTeX J Pharm Sci. Kim J, Jang JH, Lee JH, Choi JK, Park WR, Bae IH, Bae J, Park JW. Granath, K. A. (2000). Calculation of MarkHouwinkSakurada (MHS) equation viscometric constants for chitosan in any solventtemperatura system using experimental reported viscometric constants data. Biodegradable dextran hydrogels crosslinked by stereocomplex formation for the controlled release of pharmaceutical proteins. Pharmacol Pharm. Hydrodynamic J. Phys. Alternatives to animal testing: a review. Carbohydrate Polymers 99, 199-207. The basic setup of a DLS instrument is shown in Figure 1. Radius Cookies policy. ; Prasad, D.H.L. Pharm Res. All authors have consented to the submission of this manuscript for publication. Careers. Macromolecules, 29, 2265-2268. Normally, the skin loses very little water loss whereas damaged skin loses more [22]. Fusco V, Quero GM, Cho GS, Kabisch J, Meske D, Neve H, Bockelmann W, Franz CM. (1969). WebThe Stokes radius or StokesEinstein radius of a solute is the radius of a hard sphere that diffuses at the same rate as that solute. To overcome these limitations, microporation devices that act as therapeutic vehicles for delivering drug molecules across the skin barriers have been developed. Drug Deliv Syst. hydrodynamic ; Tsuchiya, H.M.; Rist, C.E. Garcia de la Torre, J.; Carrasco, B. Teixeira CG, Fusieger A, Milio GL, Martins E, Drider D, Nero LA, de Carvalho AF. Die Makromolekulare Chemie, 148(1), 229-238. The Mark-Houwink parameters is calculated molecular weights range from 8.8 to 200kDa. Thus, it is a common practice to express the frictional coefficient of rigid structures as. dextran hydrodynamic radius (2007). Increased epidermal laser fluence through simultaneous ultrasonic microporation. The Transdermal diffusion cell system (DHC-6TD, LOGAN instrument, USA) was used to assess FITC-dextran permeability in vitro. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter (d h ~ 70 nm) on order of the collagen mesh size. In order to confirm the molecular weight the gel permeation chromatography (GPC) for standard dextrans is used in a range of molecular weights from 8.8 to 2000kDa and these correspond to the following equation. These results suggest that fibroblasts proliferate and migrate under the SC, and regenerate the skin. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. Progress in Polymer Science, 30, 38-70. J Cosmet Laser Ther. Department of Biomedical-Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si, Gyeonggi-do, 14662, Republic of Korea, LG electronics, 19 Yangjae-daero 11-gil, Seocho-gu, Seoul, 06772, Republic of Korea, You can also search for this author in Dextran produced from Weissella cibaria JAG8 was purified and characterized. J. However, the currently available transdermal systems are limited by low drug permeability across the skin due to the lipophilic barrier function of the stratum corneum (SC), the outermost skin layer of the skin [3,4,5]; the methods favor low molecular mass, and lipophilic molecules, making it difficult to exploit the transdermal route for large and water-soluble molecules such as proteins and peptides. J Control Release. 43-1, 115-147. Hindered diffusion of dextran and ficoll in microporous membranes. These results are in agreement with a previous study which suggested that the microporation using radiofrequency causes disruption of the SC, enabling the percutaneous permeation of the FITC-dextran [21]. Would you like email updates of new search results? By using this website, you agree to our 113, 81-86. Intrinsic viscosity molecular weight relationship. 2012;87(6):1728. Masuelli, Martin Alberto. In our present study, microporated skin permeated insufficient amount of 20K FITC-dextran, suggesting that enhancement of permeability was unsuccessful with macromolecules of more than 20KM.W.. Universal size-independent quantities for the conformational characterization of rigid and flexible macromolecules. Soc., 16, 3A, 426-433. This study demonstrated that hydrodynamic volume and radius of gyration of a dextran sample with M w < 20 kDa and its linear counterpart with equal M w are almost The biological activity of cationic liposomes in drug delivery and toxicity test in animal models. ; Bos, G.W. These studies were again focused on investigating the hydrodynamic properties of the polymer molecules, their hydrodynamic radius in relation to their radius of gyration and the coherence of the simulation results with polymer theory. Figure3 shows a magnified image of human cadaver skin with microchannels formed by the microporation device (250kHz) and the skin closure over time. Federal government websites often end in .gov or .mil. Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. 2005;22(4):5505. Carbohydrate Polymers, 79, 380-390. The explanation for the anomalous values of a and kM-H is because the mixture solute-solvent is highly compatible due a great compaction of biopolymer, from undisturbed state in the absence of interactions, where free energy of mixing less than zero, and emphasize the nature non-linear or hyperbranched of dextran for Mw high to 200kDa. WebAccording to the above theoretical analysis for the micro-roughness contact model, the relationship between the hydrodynamic pressure (HP) and the contact pressure (CP) is shown in Fig. ; Rjumtsev, E.I. Physicochemical studies of oligodextran. Carbohydrate Polymers, 36, 319-326. Journal of Chemical Education, 80(9), 1036-1038. Static and Dynamic Light-Scattering Solution Behavior of Pullulan and Dextran in Comparison. (2008). FITC-dextran molecular weights; 4, 10, and 20kDa molecular weight were purchased from Sigma (St. Louis, MO, USA). 2022 Feb 22;16(2):1999-2012. doi: 10.1021/acsnano.1c07010. Ultimately they concluded that the temperature increment found to be an effective force in disturbing these interactions, mainly between polymer-polymer and polymer-solvent molecules for the used system. Kim, D.; Robyt, J. F.; So-Young Lee; Jin-Ha Lee; Young-Min Kim. Hi Sir Alexander Malm , Thank you very much! Really appreciate your help! A similar combination involves the intrinsic viscosity and specific volume: (a/b) is called Einstein viscosity increment, and Vs is specific volume (cm3/g). Biomaterials. Learn More. Disclaimer. These studies on Mark-Houwink parameters are usually carried out at a given temperature, obtaining a consistent result but in a very limited temperature range [68]. Stokes radius 2011;5(2):95132. Radiofrequency with microneedling. Dilute solution properties and degree of chain branching Lee WR, Shen SC, Sun CK, Aljuffali IA, Suen SY, Lin YK, Wang JJ, Fang JY. Characterization of high molecular weight dextran produced by Weissella cibaria CMGDEX3. Increased TEWL values are evidence of micropores formation. Carbohydrate Polymers 88 1440-1444. The skin culture medium was purchased from Biopredic International (Saint-Grgoire, France). Carbohydrate Research, 160, 205-214. Pribush, A.; Zilberman-Kravits, D.; Meyerstein, N. (2007). The Solomon-Ciuta single-point method is used for calculating dextran intrinsic viscosity and the error relative is calculated, and %RE respect to Huggins method is >4.18% for 2000kDa, and >2.06% for 8.8kDa (Table 2). Bethesda, MD 20894, Web Policies 2018 Aug 9;23(8):1984. doi: 10.3390/molecules23081984. Dextran. Hi Khanh, Alan is taking a little time off of Research Gate at the moment, but here is some information in reply to your question. The Mark-Houwink. Challenges for the modern analytical ultracentrifuge analysis of polysaccharides. European. The polymer molecule assumes a tighter configuration, and the solution has a lower intrinsic viscosity. 2005;304(1):27486. (2013). Structural and biocompatibility properties of dextran from 2009;6(4):34354. Chem. In addition, microporated pig skin permeated larger amounts of FITC-dextran than human skin, this can be attributed to the differences in structure between the human and pig skin; the pig skin layer is thinner (SC, 813m) than the human skin (SC, 1017m) and the pig hair follicle is longer (3871m) than the human hair follicle (18m). Int J Pharm Investig. WebTo address this perception, in the current study, FITC-dextran of different MWs (10, 70 and 150 kDa) was used to model the transdermal delivery of low MW biotherapeutics and mAbs with MWs of approximately 150 kDa. Kano S, Todo H, Furui K, Kenichi S, Tokudome Y, Hashimoto F, Kojima H, Sugibayashi K. Comparison of several reconstructed cultured human skin models by microscopic observation: their usefulness as an alternative membrane for skin in drug permeation experiments. The important role of thermodynamic interaction parameter in the determination of theta temperature, dextran/water system. 2015;23(3):2239. As a consequence of the diversity in their origin, the set of classical universal size independent quantities suffers from shortcomings. Transdermal delivery is of great importance for the effective delivery of bioactive or therapeutic agents into a body. Dextran, Tetramethylrhodamine, 2,000,000 MW, Lysine Harding, Stephen E. (1997). Hi Alan F Rawle sir, Can you help me in calculating the size of dextran 6 (molecular weight= 6000) and dextran 70 (molecular weight 70000) If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. The flattening of the curves at higher molecular weight is in agreement with an increased branching density. Dulbeccos Phosphate-Buffered Saline (DPBS) was purchased from Welgene (Gyeongsan, Korea). The value of kM-H in this work decreases with increasing temperature in contrast to published by reference [34] where kM-H increases. There is a deviation from linearity after 580kDa [69, 70]. Leiva, Angel; Muoz, Natalia; Urza, Marcela; Gargallo, Ligia; Radic, Deodato (2010). WebHydrodynamic radius = variable size of an analyte with specific MW based on dynamical properties. The values of 0 decreases from 2.5429 to 2.3326 x 1023mol-1 demonstrating a relative flexibility of particles. The dextran was chosen as another branched polysaccharide which, like the others was suspected to show hyperbranched behavior. There is a formula that used to calculate the radius (in Angstrom) from the molar mass (in g/mol) of dextran, a=0.33*(MM^0.46) where, a and MM are The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. H&E stained images were analyzed using Image J software. The Mark-Houwink a constant is close to 0.5 in poor solvents. Nanoparticles in magnetic resonance imaging: from simple to dual WebAmines per 70KDa dextran Theeffectofchargerepulsiononthe hydrodynamicradiuscanbeseenwithRh increaseswithchargedensity.Charge repulsioncausesthehydrodynamicradius toincrease.Withincreasingcharge,the dextranprogressivelyunfolded.Evena lowlevelofaminationcausesthepolymer tounfold.

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